Dynamic mass redistribution assay

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WebNov 1, 2024 · Dynamic mass redistribution label-free assay The DMR assay was performed using an Epic Benchtop System from Corning® (Corning, New York, NY; USA) and … WebOct 1, 2012 · Dynamic Mass Redistribution Assays The ReN cells were seeded in a freshly prepared laminin-coated biosensor microplate at 3000 cells per well at passages 2 to 10 suspended in 50 µL of growth factors containing medium and were cultured at 37 °C under 5% CO 2 for 2 days to reach ~90% confluency.

Demonstration of the dynamic mass redistribution label-free …

WebMay 1, 2024 · The DMR assay provides a robust and efficient platform to characterize pharmacological agents acting on adipocytes, which was used to further explore the potential activity of the bone-derived hormone osteocalcin. 2. Materials and methods 2.1. Isolation of primary preadipocytes WebThe dynamic mass redistribution (DMR) assay is a label‐free approach based on an optical biosensor technology.6,7 Using a resonant waveguide grating, DMR measures changes in the refractive index of the bottom por-tion of the cell layer. Several intracellular events can lead to changes fisher 1280x aquanaut

Applying label-free dynamic mass redistribution assay for …

WebAn example is the Epic® optical-based biosensor that relies on dynamic mass redistribution (DMR) for detection. So far, DMR assays have been mostly used to study G protein … WebStem cells hold great potential in drug discovery and development. However, challenges remain to quantitatively measure the functions of stem cells and their differentiated products. Here, we applied fluorescent imaging, quantitative real-time PCR, and label-free dynamic mass redistribution (DMR) assays to characterize the differentiation process of … WebAn optical dynamic mass redistribution assay reveals biased signaling of dualsteric GPCR activators. Anna Kebig Pharmacology and Toxicology Section, Institute of Pharmacy, ... An optical biosensor technique was applied to measure activation-induced dynamic mass redistribution (DMR) in CHO cells stably expressing the muscarinic receptor subtype ... fisher 1280x headphones

Dynamic mass redistribution as a means to measure and ... - PubMed

Dynamic Mass Redistribution Assay Decodes Differentiation of a …

WebApr 20, 2012 · Whole cell dynamic mass redistribution (DMR) assays enabled by label-free optical biosensor were then used to characterize their agonist activity in native HT-29. WebAug 3, 2011 · Dynamic mass redistribution (DMR), a label-free technology that uses light to measure ligand-induced changes in the mass of cells proximate to the biosensor, provides an integrated cellular response comprising multiple pathways and cellular events. fisher 1301 bulletinWebAug 3, 2011 · A Dynamic Mass Redistribution Assay for the Human Sweet Taste Receptor Uncovers G-Protein Dependent Biased Ligands. 17 February 2024 Frontiers in … fisher 1280x headphone pads

"WebPai, S., Verrier, F., Sun, H., Hu, H., Ferrie, A. M., Eshraghi, A., & Fang, Y. (2012). Dynamic Mass Redistribution Assay Decodes Differentiation of a Neural ... " - Dynamic mass redistribution assay

Dynamic mass redistribution assay

A New Model of Sensorial Neuron-Like Cells for HTS of Novel …

WebFeb 17, 2024 · Here, we developed a novel assay for the human sweet taste receptor where endogenous G proteins and signaling pathways are recruited by the activated receptor. The effects of several sweet taste... WebNov 1, 2024 · Introduction The label-free dynamic mass redistribution-based assay (DMR) is a powerful method for studying signalling pathways of G protein-coupled receptors (GPCRs). Herein we present the label-free DMR assay as a robust readout for pharmacological characterization of formyl peptide receptors (FPRs) in human neutrophils. Methods

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WebAug 10, 2012 · Dynamic Mass Redistribution Assay Decodes Differentiation of a Neural Progenitor Stem Cell - Sadashiva Pai, Florence Verrier, Haiyan Sun, Haibei Hu, Ann M. Ferrie, Azita Eshraghi, Ye Fang, 2012 Skip to main content Intended for healthcare professionals 0Cart MENU Search Browse Resources Authors Librarians Editors Societies Advanced … WebJan 10, 2024 · Dynamic mass redistribution as a means to measure and differentiate signaling via opioid and cannabinoid receptors Assay Drug Dev. Technol. , 9 ( 4 ) ( 2011 ) , pp. 362 - 372 CrossRef View Record in Scopus Google Scholar

WebJun 10, 2024 · Stimulation of the cells can lead to a dynamic mass redistribution (DMR) of cellular constituents and subsequently a change in refractive index. This leads to a shift of the resonant... WebLabel-free dynamic mass redistribution (DMR) is a cutting-edge assay technology that enables real-time detection of integrated cellular responses in living cells. It relies on …

WebDec 3, 2024 · Dynamic mass redistribution assay. Confluent cells were subcultured using trypsin/EDTA and used for experiments. Cells were seeded at a density of 20 000 cells/well in 30 μL into fibronectin‐coated Enspire™‐LC 384‐wells plates and cultured for 20 hours to form a confluent monolayer. On the day of the experiment, cells were manually ... WebApr 29, 2024 · (A) Dynamic mass redistribution assays quantifying phenylephrine efficacy in HEK293 cells transfected with WT, N65Q, N82Q, or NQQ SNAP-α 1D. Data are the mean of 8 replicates ± SEM; **p < 0.01 E ...

WebDec 3, 2024 · NPS modulates several biological functions including anxiety, wakefulness, pain, and drug abuse. The aim of this study was the investigation of the pharmacological profile of NPSR using the dynamic mass redistribution (DMR) assay. DMR is a label-free assay that offers a holistic view of cellular responses after receptor activation.

WebApr 12, 2024 · As one of the new generation HTS techniques, dynamic mass redistribution (DMR) assay has become an attractive approach for drug discovery [25]. It permits non-invasive, wide target/pathway coverage and multi-assay format detection for compound screening [26], and thus suitable for NPs to be excavated [27], [28]. fisher 1301f-2WebApr 11, 2024 · Dynamic mass redistribution assay. All DMR assays were performed using an Epic® BT system (Corning, NY, USA). Cells were directly seeded in Epic 384-well biosensor microplates. The seeding density of HT-29 was 32,000 cells/well. CHO–K1-hGPR35a and CHO–K1-mGPR35 were seeded at a density of 15,000 cells/well. After being cultured for … fisher 1266x proWebSep 27, 2024 · Dynamic mass redistribution (Epic) assay. Vero cells were seeded at a cell density of 5 × 10 3 cells/well in a 384-well fibronectin-coated Epic plate (Corning #5042) and grown overnight at 37°C in 5% CO 2. On the day of assay, medium was aspirated and replaced with 40 μl/well assay buffer (HBSS in 20 mM HEPES) and equilibrated at RT for 1 … fisher 1290 regulatorWebDynamic mass redistribution : a novel physiological signal of cells for cell systems biology and pharmacology Ye Fang, A. Ferrie, +6 authors R. Verkleeren Published 2007 Biology … fisher 1301f-3WebDynamic Mass Redistribution Assays The ReN cells were seeded in a freshly prepared laminin-coated biosensor microplate at 3000 cells per well at passages 2 to 10 … canada dry peach mangoWebDynamic mass redistribution is a cellular process that occurs when molecules within a cell change their intracellular locations. This phenomenon can be detected by passing polarized light... canada dry new castle deWebIntroduction: The label-free dynamic mass redistribution-based assay (DMR) is a powerful method for studying signalling pathways of G protein-coupled receptors (GPCRs). Herein … fisher 1301f manual