TīmeklisThe recombinant enzyme regiospecifically transfers a rhamnose moiety to 8-prenylkaempferol (1) and anhydroicaritin (2) at the 3-OH position to form baohuoside II (1a) and baohuoside I (2a) in vitro. In addition, a UDP-rhamnose synthase gene, EpRhS , from E. pseudowushanense was functionally characterized and used to … Tīmeklisthat Asp356, His357, Pro147 and Ile148 are key residues for sugar donor recognition and specificity for UDP-b-L-rhamnose. The mutant H357Q exhibited activity with both UDP-b-L-rhamnose and UDP-glucose. Struc-tural comparison and mutagenesis confirmed that His21 is a key residue as the catalytic base and the only
Cloning and characterization of a putative UDP-rhamnose …
Tīmeklis2016. gada 30. jūn. · Three full-length putative UDP-rhamnose: flavonoid glycoside 2″-O-beta-l-rhamnosyltransferase-like genes were isolated and designated as LcFGRT2, LcFGRT4, and LcFGRT5. Phylogenetic analysis showed that these genes were clustered with other glucoside-glycosyltransferases. Notable activities in catalyzing … Tīmeklis2024. gada 1. okt. · Cloning and characterization of a putative UDP-rhamnose synthase 1 from Populus euramericana Guinier. J. Plant Biol. (2013) P. Jones et al. … highest pc requirements game
Overexpression, purification, biochemical and structural ...
Tīmeklis2013. gada 8. febr. · L-Rhamnose is a constituent of plant primary cell wall polysaccharides including rhamnogalacturonan-I, rhamnogalacturonan-II, and other … Tīmeklis2024. gada 2. sept. · Maximal UDP-rhamnose production reached 82.2 mg/L in the recombinant strain by introducing the cellobiose phosphorolysis pathway and Arabidopsis thaliana UDP-rhamnose synthase (AtRHM). Quercitrin production of 3522 mg/L was achieved in the recombinant strain by coupling the UDP-rhamnose … Tīmeklis2024. gada 10. jūn. · A bacterial inverting glycosyltransferase EarP transfers rhamnose from dTDP-β-l-rhamnose (TDP-Rha) to Arg32 of translation elongation factor P (EF-P) to activate its function. ... Complex Structure of Pseudomonas aeruginosa Arginine Rhamnosyltransferase EarP with Its Acceptor Elongation Factor P J Bacteriol. … highest pc rated game on metacritic